Abstract:
Background: Titanium dioxide nanoparticles have various applications in biomedicine and
the food industry. Green methods for producing metal oxide nanoparticles are gaining
popularity due to their ease and environmental friendliness. Green synthesis of titanium
dioxide nanoparticles using Jatropa curcas leaf extract is a new approach to improve the
biocompatibility of the nanoparticles.
Objective: To determine the in vitro antioxidant anticoagulant antibacterial and
biocompatibility activities of titanium dioxide nanoparticles synthesized by the aqueous leaf
extract of jatropha carcus plant
Methods: The synthesis of Titanium dioxide nanoparticles was optimized using a response
surface approach, with variations in pH, temperature, and extract volume and substrate
concentration. The resulting nanoparticles were characterized using UV–Visible
spectrophotometry, FTIR Spectroscopy, and X-Ray Diffraction analysis. The biologically
synthesized titanium dioxide was tested for their antioxidant, anticoagulant, antibacterial, and
biocompatibility activities.
Results: Synthesis of titanium dioxide nanoparticle was confirmed through the absorption
band at 301 nm, and the FTIR spectra confirmed the titanium dioxide fingerprint region with
a characteristic peak at 1018 cm-1. The XRD analysis showed an average particle size of
29.01 nm for the titanium dioxide nanoparticles. At the higher concentration of 640 ppm, the
TiO2 NPs demonstrated 76.67% scavenging activity compared to the standard control
(72.71%). A dense thickened blood clot was observed in culture tube A, while no blood clot
was seen in culture tube B containing titanium dioxide nanoparticles, even after a longer
period of time. Ttitanium dioxide nanoparticle demonstrated inhibitory activity against the
test pathogenic strains Staphylococcus aureus and Escherichia coli in the following
descending order of concentration: 200 ppm > 150 ppm > 100 ppm > 50 ppm. Ttitanium
dioxide nanoparticle of a concentration of 640ppm showed 3% haemolytic activity, while a
concentration of 40ppm showed 0.4% haemolytic activity.
Conclusion: Jatropha curcas leaf extract-derived green synthesized titanium dioxide
nanoparticles offer a promising approach for biomedical applications due to their eco friendly, low-cost, and reproducible nature. They also exhibit promising bioactivities and warrant further exploration for additional biomedical applications
