Abstract:
Nanotechnology is the most promising field for generating new applications in medicine.
Green synthesis of AgNPs is an efficient, fast, cheap, non-toxic, simple, and eco-friendly than
commonly used physicochemical methods. The present study focuses on green synthesis,
characterization, antibacterial and antioxidant activities of AgNPs synthesized using
aqueous extract of Moringa stenopetala leaves. The green synthesis of AgNPs was done by
using aqueous extract of Moringa stenopetala leaves and silver nitrate solution at room
temperature. Formation of AgNPs was confirmed by the appearance of dark-brown color of
the mixture and further confirmed by spectral analysis. Parameters affecting the formation of
AgNPs were investigated and optimized. Optimum heating time required for the preparation
of aqueous extract of Moringa stenopetala leaves was 15 minutes. It was found that 90 mL of
1 mM of silver nitrate solution and 15 mL of aqueous extract of Moringa stenopetala leaves
were more favorable for maximum production of AgNPs with a yield of 97.14 %. UV-Vis
spectrum of synthesized AgNPs shows a peak at 412 nm after 24 hours of incubation time.
FTIR spectral analysis indicated the involvement of carbonyl (-C=O), hydroxyl (-O-H) and
amine (-N-H) functional groups in the formation and stabilization of AgNPs. The XRD
pattern showed the face centered cubic structure of synthesized AgNPs with average particle
size of 11.44 nm. The results of the present study revealed that synthesized AgNPs showed
stronger antibacterial activity against E. coli than S. aureus. The present study showed that
synthesized AgNPs has better antioxidant activity than standard ascorbic acid. Synthesized
AgNPs had an efficient antibacterial and antioxidant activities.
