GREEN SYNTHESIS, CHARACTERIZATION, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF SILVER NANOPARTICLES SYNTHESIZED USING AQUEOUS EXTRACT OF MORINGA STENOPETALA LEAVES

Abstract

Nanotechnology is the most promising field for generating new applications in medicine. Green synthesis of AgNPs is an efficient, fast, cheap, non-toxic, simple, and eco-friendly than commonly used physicochemical methods. The present study focuses on green synthesis, characterization, antibacterial and antioxidant activities of AgNPs synthesized using aqueous extract of Moringa stenopetala leaves. The green synthesis of AgNPs was done by using aqueous extract of Moringa stenopetala leaves and silver nitrate solution at room temperature. Formation of AgNPs was confirmed by the appearance of dark-brown color of the mixture and further confirmed by spectral analysis. Parameters affecting the formation of AgNPs were investigated and optimized. Optimum heating time required for the preparation of aqueous extract of Moringa stenopetala leaves was 15 minutes. It was found that 90 mL of 1 mM of silver nitrate solution and 15 mL of aqueous extract of Moringa stenopetala leaves were more favorable for maximum production of AgNPs with a yield of 97.14 %. UV-Vis spectrum of synthesized AgNPs shows a peak at 412 nm after 24 hours of incubation time. FTIR spectral analysis indicated the involvement of carbonyl (-C=O), hydroxyl (-O-H) and amine (-N-H) functional groups in the formation and stabilization of AgNPs. The XRD pattern showed the face centered cubic structure of synthesized AgNPs with average particle size of 11.44 nm. The results of the present study revealed that synthesized AgNPs showed stronger antibacterial activity against E. coli than S. aureus. The present study showed that synthesized AgNPs has better antioxidant activity than standard ascorbic acid. Synthesized AgNPs had an efficient antibacterial and antioxidant activities.